Limiting dilution analysis of virus-specific memory B cells by an ELISPOT assay
MK Slifka, R Ahmed - Journal of immunological methods, 1996 - Elsevier
Many acute viral infections induce long-term, sometimes even life-long humoral immunity.
To characterize this immune response, accurate quantitation of memory B cells and plasma
cells is essential. Plasma cells can be quantitated directly ex vivo by virtue of their ability to
spontaneously secrete antibody. Memory B cells on the other hand, do not spontaneously
secrete antibody but require antigenic stimulation in order to proliferate and differentiate into
antibody secreting cells (ASC). In this study, an ELISPOT-based limiting dilution assay (LDA) …
To characterize this immune response, accurate quantitation of memory B cells and plasma
cells is essential. Plasma cells can be quantitated directly ex vivo by virtue of their ability to
spontaneously secrete antibody. Memory B cells on the other hand, do not spontaneously
secrete antibody but require antigenic stimulation in order to proliferate and differentiate into
antibody secreting cells (ASC). In this study, an ELISPOT-based limiting dilution assay (LDA) …