[CITATION][C] On the chemistry and occurrence of sphingolipid long-chain bases

KA Karlsson - Chemistry and physics of lipids, 1970 - Elsevier
Methods Most of the methods used for illustration in this paper have been reported in
concise form elsewhere, and will be subjected to detailed discussion later. Sphingomyelin
fractions and ceramide aminoethylphosphonate were degraded to ceramide by
phospholipase, followed by alkaline hydrolysis and column chromatographic preparation of
long-chain base hydrochlorides zs). Long-chain base hydrochlorides were converted to their
corresponding dinitrophenyl (DNP) derivatives 26) in the following way and proportions …