[HTML][HTML] Preparation and culture of bone marrow-derived macrophages from mice for functional analysis
G Toda, T Yamauchi, T Kadowaki, K Ueki - STAR protocols, 2021 - Elsevier
G Toda, T Yamauchi, T Kadowaki, K Ueki
STAR protocols, 2021•ElsevierThe assessment of macrophage function has been a topic of intense discussion due to
multiple subtypes. This protocol describes the collection of bone marrow cells from the femur
and tibia of mice, differentiation into bone marrow-derived macrophages (BMDM cells), and
sampling from cultures. This protocol focuses on the efficient preparation of BMDM cells,
providing a way to assess the function of macrophages. For complete details on the use and
execution of this protocol, please refer to Toda et al.(2020).
multiple subtypes. This protocol describes the collection of bone marrow cells from the femur
and tibia of mice, differentiation into bone marrow-derived macrophages (BMDM cells), and
sampling from cultures. This protocol focuses on the efficient preparation of BMDM cells,
providing a way to assess the function of macrophages. For complete details on the use and
execution of this protocol, please refer to Toda et al.(2020).
Summary
The assessment of macrophage function has been a topic of intense discussion due to multiple subtypes. This protocol describes the collection of bone marrow cells from the femur and tibia of mice, differentiation into bone marrow-derived macrophages (BMDM cells), and sampling from cultures. This protocol focuses on the efficient preparation of BMDM cells, providing a way to assess the function of macrophages.
For complete details on the use and execution of this protocol, please refer to Toda et al. (2020).
Elsevier