Molecular identification of bacterial 16S ribosomal RNA gene in liver tissue of primary biliary cirrhosis: is Propionibacterium acnes involved in granuloma formation?
K Harada, K Tsuneyama, Y Sudo, S Masuda… - Hepatology, 2001 - Elsevier
The etiopathogenesis of primary biliary cirrhosis (PBC) remains speculative. Epithelioid
granulomas are often found in the vicinity of damaged interlobular bile ducts in PBC, raising
the possibility of a reaction to microbial materials. In this study, we tried to detect and identify
bacterial DNA within granulomatous lesions in PBC. Using liver sections from 9 patients with
PBC and 13 control livers, granuloma in portal tracts, portal tracts without granuloma, and
adjacent hepatic parenchyma were selectively microdissected from sections, and then DNA …
granulomas are often found in the vicinity of damaged interlobular bile ducts in PBC, raising
the possibility of a reaction to microbial materials. In this study, we tried to detect and identify
bacterial DNA within granulomatous lesions in PBC. Using liver sections from 9 patients with
PBC and 13 control livers, granuloma in portal tracts, portal tracts without granuloma, and
adjacent hepatic parenchyma were selectively microdissected from sections, and then DNA …
