Multiplex quantitative polymerase chain reaction assay for the identification and quantitation of major vaginal lactobacilli
SV Balashov, E Mordechai, ME Adelson… - … and infectious disease, 2014 - Elsevier
Lactobacilli play a key role in promoting vaginal health. Depletion of these bacteria is
associated with bacterial vaginosis (BV), the most common vaginal disorder. Here we
describe the development and laboratory validation of a novel single-tube multiplex TaqMan
quantitative polymerase chain reaction (qPCR) assay for the identification and quantitative
assessment of the four major vaginal Lactobacillus species: L. crispatus, L. jensenii, L.
gasseri, and L. iners. The assay utility was evaluated by the analysis of lactobacilli in non …
associated with bacterial vaginosis (BV), the most common vaginal disorder. Here we
describe the development and laboratory validation of a novel single-tube multiplex TaqMan
quantitative polymerase chain reaction (qPCR) assay for the identification and quantitative
assessment of the four major vaginal Lactobacillus species: L. crispatus, L. jensenii, L.
gasseri, and L. iners. The assay utility was evaluated by the analysis of lactobacilli in non …