[PDF][PDF] The isolation and crystallization of human insulin
IA Mirsky, R Jinks, G Perisutti - The Journal of Clinical …, 1963 - Am Soc Clin Investig
IA Mirsky, R Jinks, G Perisutti
The Journal of Clinical Investigation, 1963•Am Soc Clin InvestigMETHODS The human pancreases utilized in this study were obtained at autopsy atvarious
undetermined intervals after death, frozen as rapidly as possible, and stored in a deep
freezer until ready for use. The frozen pancreases were extracted by either the acid-alcohol
procedure (4, 5) or the salt-alcohol procedure described by Romans (7, 8). Since preliminary
experiments suggested thatthe latter procedure, with minor modifications, resulted in a
greater yield, it was used in most of the studies conducted dur-ing the past 2 years.
undetermined intervals after death, frozen as rapidly as possible, and stored in a deep
freezer until ready for use. The frozen pancreases were extracted by either the acid-alcohol
procedure (4, 5) or the salt-alcohol procedure described by Romans (7, 8). Since preliminary
experiments suggested thatthe latter procedure, with minor modifications, resulted in a
greater yield, it was used in most of the studies conducted dur-ing the past 2 years.
METHODS
The human pancreases utilized in this study were obtained at autopsy atvarious undetermined intervals after death, frozen as rapidly as possible, and stored in a deep freezer until ready for use. The frozen pancreases were extracted by either the acid-alcohol procedure (4, 5) or the salt-alcohol procedure described by Romans (7, 8). Since preliminary experiments suggested thatthe latter procedure, with minor modifications, resulted in a greater yield, it was used in most of the studies conducted dur-ing the past 2 years.
The Journal of Clinical Investigation