Single-cell analysis has become an method of importance in immunology. Fluorescence
flow cytometry has been a major player. However, due to issues such as autofluorescence
and emission spillover between different fluorophores, alternative techniques are being
developed. In recent years, mass cytometry has emerged, wherein antibodies labeled with
metal ions are detected by ICP-MS. In order for a cell to be seen, a metal in the mass
window must be present; there is no analogous parameter to forward or side scatter. The …